Plenary Lectures

Opening Plenary Speaker

HoodDr. Leroy Hood
President & Co-Founder, Institute for Systems Biology


"Technologies and Strategies for Driving the Personalize Medicine Revolution: Transforming Healthcare through Wellness"

 

Dr. Leroy E. Hood graduated from the Johns Hopkins University School of Medicine in 1964 with an MD and from Caltech with a PhD in biochemistry in 1968. After three years as a Senior Investigator at NIH, his academic career began at Caltech, where he and his colleagues developed the DNA gene sequencer and synthesizer, and the protein synthesizer and sequencer–four instruments that paved the way for the successful mapping and understanding of the human genome. A pillar in the biotechnology field, Dr. Hood has played a role in founding fifteen biotechnology companies including Amgen, Applied Biosystems, Integrated Diagnostics and Arivale. He is a member of the National Academy of Sciences, the National Academy of Engineering, and the Institute of Medicine. Of the more than 6,000 scientists world-wide who belong to one or more of these academies, Dr. Hood is one of only fifteen people nominated to all three. Dr. Hood has co-authored numerous textbooks in biochemistry, immunology, molecular biology and genetics, as well as a popular book on the human genome project, The Code of Codes and he is just finishing up a text on systems biology. He is the recipient of numerous national and international awards, including the Lasker Award for Studies of Immune Diversity (1987), the Kyoto Prize in advanced technology (2002), the Heinz Award for pioneering work in Systems Biology (2006), and the coveted NAE 2011 Fritz J. and Delores H. Russ Prize for developing automated DNA sequencing. In addition to having received 17 honorary degrees from prestigious universities in the U.S. and abroad, Dr. Hood has published over 750 peer reviewed articles and currently holds 36 patents. In 2013, he received the National Medal of Science from President Obama. Hood has been named by The Best Schools as one of the 50 Most Influential Scientists in the World Today (2014). Scientific American has named Hood as one of the top 6 in their selection of 100 biotech visionaries world-wide (2015).

Opening Plenary Speaker

 

CarrDr. Steven Carr
Director, Broad Institute of MIT and Harvard


"Advancing Biology, Chemistry and Medicine through High-Resolution Liquid  Chromatography Mass Spectrometry-based Proteomics"


Steven Carr is Director of Proteomics at the Broad Institute of MIT and Harvard. He is internationally recognized as a leader in the development of novel proteomics methods and in their application in biology and medicine.  Steve and his group collaborate with scientists throughout the Broad Institute community  (Harvard University, Harvard Medical School, and the 17 Harvard-affiliated hospitals) to apply state-of-the-art proteomics technology to address compelling questions in biology, chemistry, and clinical medicine. Research in his lab focuses on detecting and quantifying post-translational modifications (e.g., phosphorylation, acetylation, ubiquitylation, methylation, etc.) in the proteome, developing new technologies to quantify proteins in cells and biofluids with high sensitivity and specificity, improving informatics for peptide and protein assignment using mass spectrometry (MS) data and integrating MS-derived data with genomic data. The Carr group is also known for developing and applying systematic approaches for biomarker discovery and validation to ultimately provide biomarkers for cancers, heart disease, infectious diseases, and metabolic disorders. He has over 250 publications on the development and use of proteomics and biological mass spectrometry.

Opening Plenary Speaker

 

Dr. Mary Wirth

W. Brooks Fortune Distinguished Professor, Purdue University


"UHPLC of Therapeutic Monoclonal Antibodies using Submicrometer Particles"

 

We work at the interface of chemistry and medicine, and our focus is to create technology for earlier detection of diseases. The dream of 21st century medicine is that simple lab tests will reveal diseases well before the onset of symptoms, while the disease is easily curable. We are using nanotechnology to modernize the materials used for lab tests and for the discovery of the biomarkers that are the targets of lab tests. An area of interest is capillary columns for nanoUPLC that are packed with silica nanoparticles. The packing is an artificial opal, which exhibits a Bragg diffraction due to the crystalline packing of the nanoparticles. The scanning electron micrograph of the capillary cross-section shows the face-centered cubic packing. This highly ordered packing imparts extraordinary separation efficiency and speed. The ability to form these face-centered cubic structures in capillaries facilitates mass spectrometric detection of proteins. We are using these materials for a range of tools used for biomarker discovery and medical tests, including capillary chromatography with nanospray MS detection, high-throughput, lab-on-a-chip devices, and basic research on biomolecule transport in nanoscale media. We are collaborating with medical researchers and industrial scientists to maximize the impact of our research.

 

Closing Plenary Speaker


Dr. Barry Karger

Director, Barnett Institute, Northeastern University


"Separation Science and Analytical Chemistry: Past, Present and Future"

 

For over 50 years, Barry L. Karger has been an active contributor to the fields of chromatography and analytical chemistry. He is the James L. Waters Chair in Analytical Chemistry and founding director of the Barnett Institute of Chemical and Biological Analysis. The institute is an internationally recognized center of excellence in protein and proteomic analysis in the clinical and biotechnology fields. Dr. Karger began his research in gas chromatography and was an early contributor to the emerging field of HPLC in the 1970s, focusing on theory and chemically bonded phases. He then advanced on protein separation by reversed phase HPLC and introduced hydrophobic interaction chromatography. He was an important contributor to the success of the Human Genome Project being the first to demonstrate the potential of capillary electrophoresis for DNA separation and the use of replaceable polymer matrices. More recently, he has focused on LC–MS and CE-MS for the characterization of protein biopharmaceuticals. In addition, he developed porous layer open tubular (PLOT) columns coupled to MS for deep proteomic analysis of only a few hundred cells. He has been widely recognized for his contributions including three American Chemical Society awards and the Bergman, Heyrovsky, Beckman and Widmer Medals.

 

Closing Plenary Speaker

 

Prof. Doo Soo Chung

Faculty, Seoul National University


"Photophoresis: Optical Force Chromatography Separating Molecules using Mechanical Forces of Light"

 

Doo Soo Chung is a professor of analytical chemistry at the Department of Chemistry, Seoul National University, where he obtained a BS degree in 1981 and an MS degree in 1983 majoring in theoretical chemistry. Then he obtained an AM degree in physics in 1986 and a PhD degree in 1991 in chemical physics at Harvard University, working on nonequilibrium liquid interfaces. During his postdoctoral research at MIT and Harvard Medical School, Chung worked on biophysics. After being appointed as an assistant professor at Seoul National University, Chung got one year training in analytical chemistry at Iowa State University. Afterwards, he has been working on a variety of topics ranging from chemical physics to bioanalytical chemistry. His group has demonstrated molecule optics that controls the molecular motions by mechanical forces of light, especially optical force chromatography separating neutral molecules. Another major research area is to improve the sensitivity of capillary electrophoresis, especially by using in-line coupled microextraction techniques for sample cleanup and preconcentration. Few examples are single drop microextraction, in-tube microextraction, and single bubble microextraction.

 

Closing Plenary Speaker

 

Dovichi Dr. Norman Dovichi

Grace-Rupley Professor of Chemistry and Biochemistry, University of Notre Dame


"Ultrasensitive Protein Analysis using Capillary Zone Electrophoresis Coupled with Tandem Mass Spectrometry"

 

Norman Dovichi is the Grace-Rupley Professor of Chemistry and Biochemistry at the University of Notre Dame. The early stages of his research career were primarily focused on development of capillary electrophoresis and ultrasensitive laser-induced fluorescence for ultrasensitive bioanalysis. In the 1980s, he introduced the concept of single molecule detection to the chemical literature. In the 1990s, his group developed capillary array electrophoresis instruments for high-throughput DNA sequencing. This technology was commercialized as the Applied Biosystems model 3700 DNA sequencer. He was recognized for this work by the journal Science as an “Unsung Hero of the Human Genome Project”. Over the last decade, his group has focused its attention on coupling capillary electrophoresis with tandem mass spectrometry as a tool for high throughput and high sensitivity proteomics. Recent results include detection of 10,000 peptides in an 80-min single-shot separation of the HeLa proteome, detection of 2,300 phosphopeptides in a 100-min single shot separation, and detection of low zeptomole amounts of tryptic peptides in complex mixtures. Finally, his group used HPLC-MS/MS for the first proteomic analysis of vertebrate development. Over 5,500 proteins were quantified at six stages of early development of Xenopus laevis. The expression profile of these proteins naturally fell into clusters that matched with the known biology of this organism. Studies also compared expression differences between single embryos, including single freshly fertilized zygotes; this study is the first comprehensive proteomic analysis of protein expression in a single cell.

 

Closing Plenary Speaker

 

Prof. Michal Holčapek 

Faculty of Chemical Technology, University of Pardubice

 

"Cancer Lipidomics: Analysis of Dysregulated Lipids using Mass Spectrometry"

 

Michal Holčapek is a full professor of analytical chemistry and a head of mass spectrometry group at the Department of Analytical Chemistry, University of Pardubice, where he has obtained Ph.D. in analytical chemistry in 1999. His research started with the structural analysis of organic and organometallic compounds using mass spectrometry (MS) and its coupling with liquid chromatography, focused mainly on the metabolism of various pharmaceutical drugs and natural compounds. The present specialization is the lipidomic analysis of biological samples using liquid chromatography or supercritical fluid chromatography coupled to MS, shotgun ESI-MS, MALDI-MS and mass spectrometry imaging. The main emphasis is on the clinical research with the goal to find biomarkers for cancers and other serious human diseases. He coauthored over 110 papers in peer-reviewed journals with h-index 32 and over 300 conference presentations including more than 20 plenary or keynote lectures. He is a member of editorial boards of Analytical Chemistry, Analytical and Bioanalytical Chemistry, Lipids, guest editor of special volumes on Mass spectrometry – innovation and application in Journal of Chromatography A (2010, 2012 and 2016), editor of special issues on Lipidomics in Analytical Chemistry (2014) and Analytical and Bioanalytical Chemistry (2015), editor of the book Extreme Chromatography: Faster, Hotter, Smaller (American Oil Chemical Society, 2011), head of Czech Mass Spectrometry Section and national representative in International Mass Spectrometry Foundation (2005-2013), chairman of several international and national conferences and stated on Power List of 100 most influential people in the analytical sciences (The Analytical Scientist, 2013 and 2015).